Review

anti-cd44v10 (Novus Biologicals)

Novus Biologicals is a verified supplier

Novus Biologicals manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Novus Biologicals anti-cd44v10

Characterization of potential bioactive components in HA-induced EV. Panel (a): HPMVEC were grown to confluence and switched to serum-free media and either no HA (control), 100 nM HMW-HA, 100 nM LMW-HA, or 500 ng/mL LPS for 24 hours. EVs were then isolated, run on 4–20% TBE gels, and stained with Alcian blue. Purified enlargeosomes contained HA of ~1 million Da (see arrow) consistent with HMW-HA which we have previously demonstrated to be barrier enhancing [ , , , ]. In contrast, control, LMW-HA, or LPS-induced EV contained negligible HA. Human plasma was used as a control. Panel (b): HPMVEC were grown to confluence and switched to serum-free media and either no HA (control), 100 nM HMW-HA, or 100 nM LMW-HA for 24 hours. EVs were then isolated, run on SDS-PAGE, and immunoblotted with anti-CD44 (IM-7) (a), <t>anti-CD44v10</t> (b), anti-HABP2 (c), anti-CD63 (d), or anti-AHNAK (e) antibodies. HMW-HA-induced enlargeosomes expressed the EC barrier enhancing CD44 isoform, CD44s (standard form) [ , ]. In contrast, LMW-HA-induced exosomes expressed the EC barrier disrupting HA binding proteins, CD44 isoform CD44v10, and the extracellular serine protease, HABP2 [ , ]. Basally secreted EV (control) had low expression of these molecules. Panel (c): isolated EVs as described in Panel (b) were subjected to RNA isolation and analysis (see ). Compared to control EV, LMW-HA-induced EV had less total RNA and microRNA while HMW-HA-induced enlargeosomes had ~2-fold higher levels of total RNA and microRNA.

Anti Cd44v10, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+cd44v10/pmc04581561-38-37-38?v=Novus+Biologicals
Average 90 stars, based on 1 article reviews

anti-cd44v10 - by Bioz Stars, 2026-08

90/100 stars

Images

1) Product Images from "Extracellular Vesicles from Caveolin-Enriched Microdomains Regulate Hyaluronan-Mediated Sustained Vascular Integrity"

Article Title: Extracellular Vesicles from Caveolin-Enriched Microdomains Regulate Hyaluronan-Mediated Sustained Vascular Integrity

Journal: International Journal of Cell Biology

doi: 10.1155/2015/481493

Figure Legend Snippet: Characterization of potential bioactive components in HA-induced EV. Panel (a): HPMVEC were grown to confluence and switched to serum-free media and either no HA (control), 100 nM HMW-HA, 100 nM LMW-HA, or 500 ng/mL LPS for 24 hours. EVs were then isolated, run on 4–20% TBE gels, and stained with Alcian blue. Purified enlargeosomes contained HA of ~1 million Da (see arrow) consistent with HMW-HA which we have previously demonstrated to be barrier enhancing [ , , , ]. In contrast, control, LMW-HA, or LPS-induced EV contained negligible HA. Human plasma was used as a control. Panel (b): HPMVEC were grown to confluence and switched to serum-free media and either no HA (control), 100 nM HMW-HA, or 100 nM LMW-HA for 24 hours. EVs were then isolated, run on SDS-PAGE, and immunoblotted with anti-CD44 (IM-7) (a), anti-CD44v10 (b), anti-HABP2 (c), anti-CD63 (d), or anti-AHNAK (e) antibodies. HMW-HA-induced enlargeosomes expressed the EC barrier enhancing CD44 isoform, CD44s (standard form) [ , ]. In contrast, LMW-HA-induced exosomes expressed the EC barrier disrupting HA binding proteins, CD44 isoform CD44v10, and the extracellular serine protease, HABP2 [ , ]. Basally secreted EV (control) had low expression of these molecules. Panel (c): isolated EVs as described in Panel (b) were subjected to RNA isolation and analysis (see ). Compared to control EV, LMW-HA-induced EV had less total RNA and microRNA while HMW-HA-induced enlargeosomes had ~2-fold higher levels of total RNA and microRNA.

Techniques Used: Isolation, Staining, Purification, SDS Page, Binding Assay, Expressing

Image Search Results

Characterization of metaplastic glands. ( A ) IF staining for TFF2 in the corpus of 6-month-old WT mice. ( B ) IF staining for Ki67, TFF2, and E-cad in the corpus of 6-month-old RUNX3 R122C/R122C mice. ( C ) GSEA showing enrichment of TFF2 targets up signature in the 6-month-old RUNX3 R122C/R122C corpus tissue compared with the 6-month-old WT tissue ( n = 3 mice each). ( D ) qPCR for Tff2 , Cd44 , and Gkn3 expression levels in the corpus tissues of 6-month-old WT and RUNX3 R122C/R122C mice ( n = 3 mice each). ( E ) IF staining for GSII and GIF in the corpus of 6-month-old RUNX3 R122C/R122C mice. ( F and G ) IF staining for TFF2, CD44v10, and GSII in the corpus tissues of 6-month-old ( F ) WT and ( G ) RUNX3 R122C/R122C mice. ( H ) The relative expression of Wfdc2 , Cftr , Gpx2 , and Dmbt1 from RNA-seq data of the corpus tissues of 6-month-old WT and RUNX3 R122C/R122C mice ( n = 3 mice each). Scale bars : 100 μm. Box indicates enlarged region. Data are presented as the means ± SEM and were analyzed by the Student t test. ∗ P < .05, ∗∗∗ P < .001. FPKM, fragments per kilobase of exon per million reads mapped. DAPI, 4’,6-diamidino-2-phenylindole.

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: A Point Mutation R122C in RUNX3 Promotes the Expansion of Isthmus Stem Cells and Inhibits Their Differentiation in the Stomach

doi: 10.1016/j.jcmgh.2022.01.010

Figure Lengend Snippet: Characterization of metaplastic glands. ( A ) IF staining for TFF2 in the corpus of 6-month-old WT mice. ( B ) IF staining for Ki67, TFF2, and E-cad in the corpus of 6-month-old RUNX3 R122C/R122C mice. ( C ) GSEA showing enrichment of TFF2 targets up signature in the 6-month-old RUNX3 R122C/R122C corpus tissue compared with the 6-month-old WT tissue ( n = 3 mice each). ( D ) qPCR for Tff2 , Cd44 , and Gkn3 expression levels in the corpus tissues of 6-month-old WT and RUNX3 R122C/R122C mice ( n = 3 mice each). ( E ) IF staining for GSII and GIF in the corpus of 6-month-old RUNX3 R122C/R122C mice. ( F and G ) IF staining for TFF2, CD44v10, and GSII in the corpus tissues of 6-month-old ( F ) WT and ( G ) RUNX3 R122C/R122C mice. ( H ) The relative expression of Wfdc2 , Cftr , Gpx2 , and Dmbt1 from RNA-seq data of the corpus tissues of 6-month-old WT and RUNX3 R122C/R122C mice ( n = 3 mice each). Scale bars : 100 μm. Box indicates enlarged region. Data are presented as the means ± SEM and were analyzed by the Student t test. ∗ P < .05, ∗∗∗ P < .001. FPKM, fragments per kilobase of exon per million reads mapped. DAPI, 4’,6-diamidino-2-phenylindole.

Article Snippet: Paraffin and frozen tissue sections were stained with the following primary antibodies: rat anti-CD45 (1:500, 14-0451-82; eBioscience, San Diego, CA), rat anti–E-cadherin (1:500, ab11512; Abcam, Cambridge, UK), mouse anti–E-cadherin Alexa Fluor 488/555/647 conjugate (1:200, 560061/560064/560062; BD Biosciences, San Jose, CA), mouse anti–H,K-ATPase α subunit (1:1000, D031-3; MBL International, Woburn, MA), anti-lectin GSII Alexa Fluor 488/647 conjugate (1:1000, L-21415/L-32451; Molecular Probes, Eugene, OR), rabbit anti-GIF (1:2000, provided by D.H. Alpers, Washington University School of Medicine, St. Louis, MO), goat anti-Muc5ac (1:200, sc-16903; Santa Cruz Biotechnology, Santa Cruz, CA), goat anti– chromogranin A (1:500, sc-1488; Santa Cruz Biotechnology), rat anti-Ki67 (1:1000, 14-5698-82; Thermo Fisher Scientific), rabbit anti-TFF2 (1:100, 13681-1-AP; Proteintech, Rosemont, IL), rabbit anti-Sox9 (1:1000, AB5535; Merck Millipore), rat anti-CD44v10 (1:500, LKG-M002; Cosmo Bio, Tokyo, Japan), rabbit anti-PDX1 (1:200, ab47267; Abcam), rabbit anti-Red fluorescent protein (1:500, PM005; MBL International), mouse anti-pRb (1:200, sc-102; Santa Cruz Biotechnology), rabbit anti–phospho-pRb (1:200, 8516; Cell Signaling Technology, Danvers, MA), rabbit anti-MCM2 (1:400, 3619; Cell Signaling Technology), rat anti-CD68 (1:200, MA5-16674; Thermo Fisher Scientific), rat anti-F4/80 (1:200, MCA497; Bio-Rad, Hercules, CA), rabbit anti-CD163 (1:100, ab182422; Abcam), rabbit anti–villin (1:100, ab130751; Abcam), rabbit anti-Muc2 (1:500, sc-15334; Santa Cruz Biotechnology), rabbit anti-CDX2 (1:100, MA5-14494; Thermo Fisher Scientific), rabbit anti-CDX1 (1:200, NBP1-49538; Novus Biologicals, Centennial, CO), rabbit anti-IQGAP3 (1:200, provided by Sachiko Tsukita, Osaka University, Osaka, Japan).

Techniques: Staining, Expressing, RNA Sequencing Assay

Characterization of metaplastic glands by using antral markers. ( A ) IF staining for Ki67 and TFF2 in the corpus of 6-month-old RUNX3 R122C/R122C mice. ( B and C ) IF staining for E-cad and Sox9 in the corpus of 6-month-old ( B ) WT and ( C ) RUNX3 R122C/R122C mice. ( D ) IF staining for TFF2, CD44v10, and GSII in the antrum of WT mice. IF staining for PDX1 in the ( E ) small intestine, antrum, and corpus of WT mice, and in the ( F ) corpus of 6-month-old RUNX3 R122C/R122C mice. ( G ) qPCR for Pdx1 expression levels in the corpus tissues of 6-month-old WT and RUNX3 R122C/R122C mice ( n = 3 mice each). Scale bars : 100 μm ( A ), 50 μm ( B–F ). Data are presented as the means ± SEM and were analyzed by the Student t test. ∗ P < .05. DAPI, 4’,6-diamidino-2-phenylindole.

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: A Point Mutation R122C in RUNX3 Promotes the Expansion of Isthmus Stem Cells and Inhibits Their Differentiation in the Stomach

doi: 10.1016/j.jcmgh.2022.01.010

Figure Lengend Snippet: Characterization of metaplastic glands by using antral markers. ( A ) IF staining for Ki67 and TFF2 in the corpus of 6-month-old RUNX3 R122C/R122C mice. ( B and C ) IF staining for E-cad and Sox9 in the corpus of 6-month-old ( B ) WT and ( C ) RUNX3 R122C/R122C mice. ( D ) IF staining for TFF2, CD44v10, and GSII in the antrum of WT mice. IF staining for PDX1 in the ( E ) small intestine, antrum, and corpus of WT mice, and in the ( F ) corpus of 6-month-old RUNX3 R122C/R122C mice. ( G ) qPCR for Pdx1 expression levels in the corpus tissues of 6-month-old WT and RUNX3 R122C/R122C mice ( n = 3 mice each). Scale bars : 100 μm ( A ), 50 μm ( B–F ). Data are presented as the means ± SEM and were analyzed by the Student t test. ∗ P < .05. DAPI, 4’,6-diamidino-2-phenylindole.

Techniques: Staining, Expressing

Figure 1. CD44v10 is preferentially overexpressed in TNBC tissues. A, CD44v10 expression in BrCa tissues and adjacent normal tissues is shown by immunohistochemistry (IHC). Representative IHC images (left) and analysis of expressions (right) for CD44v10 in 25 pairs of BrCa and matched peritumoral tissues. Statistically signiﬁcant differences were determined using t-tests. B, representative IHC images (left) and analysis of expression (right) for CD44v10 in a tissue microarray, including luminal BrCas (n = 30), BrCa patients with HER2 overexpression (n = 13), and TNBCs (n = 28). The mean of integrated optical density (IOD) was calculated by the IOD divided by the valid area. Bars represent the mean ± SD values. The ns indicates no signiﬁcance, *p <0.05, ***p <0.001. TNBCs, triple-negative breast cancers.

Journal: The Journal of biological chemistry

Article Title: Cell adhesion molecule CD44v10 promotes stem-like properties in triple-negative breast cancer cells via glucose transporter GLUT1-mediated glycolysis.

doi: 10.1016/j.jbc.2022.102588

Figure Lengend Snippet: Figure 1. CD44v10 is preferentially overexpressed in TNBC tissues. A, CD44v10 expression in BrCa tissues and adjacent normal tissues is shown by immunohistochemistry (IHC). Representative IHC images (left) and analysis of expressions (right) for CD44v10 in 25 pairs of BrCa and matched peritumoral tissues. Statistically signiﬁcant differences were determined using t-tests. B, representative IHC images (left) and analysis of expression (right) for CD44v10 in a tissue microarray, including luminal BrCas (n = 30), BrCa patients with HER2 overexpression (n = 13), and TNBCs (n = 28). The mean of integrated optical density (IOD) was calculated by the IOD divided by the valid area. Bars represent the mean ± SD values. The ns indicates no signiﬁcance, *p <0.05, ***p <0.001. TNBCs, triple-negative breast cancers.

Article Snippet: Then, a mouse anti-human CD44v10 (1:200, Bio-Rad, MCA1733) antibody was added, and the slides were incubated at 4 C overnight.

Techniques: Expressing, Immunohistochemistry, Microarray, Over Expression

Figure 2. Analysis of transcriptional changes upon CD44v10 overexpression. A, CD44v10 expression levels of human normal breast epithelial cells (MCF10A) and TNBC cells (MDA-MB-468, Hs578t, BT-549 and MDA-MB-231) were analyzed by Western blotting. β-actin was used as the loading control. B, CD44 expression levels of TNBC cells after CD44v10 siRNA transfection were analyzed by Western blotting. C, overexpression (OE) efﬁciency of CD44v10 was evaluated by Western blotting. D, volcano plot of log2 fold changes versus -log10 q value shows transcriptional differences between BT-549 vector and BT- 549 CD44v10 overexpression cells. Vertical lines represent the 1.5-fold change cut off and the horizontal lines indicate the 0.05 q value cut-off. Upregulated and downregulated genes are highlighted in red and green, respectively. E, gene ontology (GO) analysis of upregulated differentially expressed genes in terms of biological processes. F, gene set enrichment analysis (GSEA) enrichment plots of the hallmark of glycolysis and canonical glycolysis gene sets in BT- 549 CD44v10 overexpression cells compared with BT-549 vector group. FDR, false discovery rate, NES, normalized enrichment score; TNBCs, triple-negative breast cancers.

Journal: The Journal of biological chemistry

Article Title: Cell adhesion molecule CD44v10 promotes stem-like properties in triple-negative breast cancer cells via glucose transporter GLUT1-mediated glycolysis.

doi: 10.1016/j.jbc.2022.102588

Figure Lengend Snippet: Figure 2. Analysis of transcriptional changes upon CD44v10 overexpression. A, CD44v10 expression levels of human normal breast epithelial cells (MCF10A) and TNBC cells (MDA-MB-468, Hs578t, BT-549 and MDA-MB-231) were analyzed by Western blotting. β-actin was used as the loading control. B, CD44 expression levels of TNBC cells after CD44v10 siRNA transfection were analyzed by Western blotting. C, overexpression (OE) efﬁciency of CD44v10 was evaluated by Western blotting. D, volcano plot of log2 fold changes versus -log10 q value shows transcriptional differences between BT-549 vector and BT- 549 CD44v10 overexpression cells. Vertical lines represent the 1.5-fold change cut off and the horizontal lines indicate the 0.05 q value cut-off. Upregulated and downregulated genes are highlighted in red and green, respectively. E, gene ontology (GO) analysis of upregulated differentially expressed genes in terms of biological processes. F, gene set enrichment analysis (GSEA) enrichment plots of the hallmark of glycolysis and canonical glycolysis gene sets in BT- 549 CD44v10 overexpression cells compared with BT-549 vector group. FDR, false discovery rate, NES, normalized enrichment score; TNBCs, triple-negative breast cancers.

Article Snippet: Then, a mouse anti-human CD44v10 (1:200, Bio-Rad, MCA1733) antibody was added, and the slides were incubated at 4 C overnight.

Techniques: Over Expression, Expressing, Western Blot, Control, Transfection, Plasmid Preparation

Figure 3. Regulation of CD44v10 impacts glycolytic processes of TNBC cells. A, knockdown efﬁciency of CD44v10 in MDA-MB-231 cells was evaluated by Western blotting. B, analysis of glucose consumption in cells cultured for 12 and 24 h. The means ± SD of relative fold changes from triplicate ex- periments were plotted. C, analysis of lactate production in cells cultured for 12 and 24 h. D, cells were stained with 2’,7’- dichlorodihydroﬂuorescein- diacetate (DCFH-DA), and then subjected to ﬂow cytometry analysis to measure the cellular ROS level. The mean relative ﬂuorescence intensity (RFI) values are shown. *p <0.05, **p <0.01, ***p <0.001. ROS, reactive oxygen species; TNBCs, triple-negative breast cancers.

Journal: The Journal of biological chemistry

Article Title: Cell adhesion molecule CD44v10 promotes stem-like properties in triple-negative breast cancer cells via glucose transporter GLUT1-mediated glycolysis.

doi: 10.1016/j.jbc.2022.102588

Figure Lengend Snippet: Figure 3. Regulation of CD44v10 impacts glycolytic processes of TNBC cells. A, knockdown efﬁciency of CD44v10 in MDA-MB-231 cells was evaluated by Western blotting. B, analysis of glucose consumption in cells cultured for 12 and 24 h. The means ± SD of relative fold changes from triplicate ex- periments were plotted. C, analysis of lactate production in cells cultured for 12 and 24 h. D, cells were stained with 2’,7’- dichlorodihydroﬂuorescein- diacetate (DCFH-DA), and then subjected to ﬂow cytometry analysis to measure the cellular ROS level. The mean relative ﬂuorescence intensity (RFI) values are shown. *p <0.05, **p <0.01, ***p <0.001. ROS, reactive oxygen species; TNBCs, triple-negative breast cancers.

Article Snippet: Then, a mouse anti-human CD44v10 (1:200, Bio-Rad, MCA1733) antibody was added, and the slides were incubated at 4 C overnight.

Techniques: Knockdown, Western Blot, Cell Culture, Staining, Cytometry

Figure 4. CD44v10 facilitates glycolysis by upregulating GLUT1 expression. A, heatmap shows expression proﬁles of the glycolysis-related genes in BT- 549 CD44v10 overexpression cells compared to BT-549 vector cells. Red colors indicate upregulation, and blue indicates downregulation. B, expression validation of candidate genes from heatmap by qPCR. The means ± SD of relative fold changes from triplicate experiments were plotted. β-actin was used as the control. The p values were calculated by paired Student’s t test. C, analysis of GLUT1 expression by Western blot in CD44v10 OE cells. D, knockdown efﬁciency of GLUT1 was evaluated by Western blotting in CD44v10 OE cells. E, the effects of GLUT1 on glucose consumption and lactate production in BT- 549 cells. F, the effects of GLUT1 on ROS levels in BT-549 cells. G, relative levels of phosphorylation of ERK, AKT, and GLUT1 proteins in BT-549 CD44v10 overexpression cells pretreated with ERK (U0126, 20 μM) or AKT inhibitor (LY294002, 20 μM). The ns indicates no signiﬁcance, **p <0.01, ***p <0.001. GLUT1, glucose transporter 1; ROS, reactive oxygen species.

Journal: The Journal of biological chemistry

Article Title: Cell adhesion molecule CD44v10 promotes stem-like properties in triple-negative breast cancer cells via glucose transporter GLUT1-mediated glycolysis.

doi: 10.1016/j.jbc.2022.102588

Figure Lengend Snippet: Figure 4. CD44v10 facilitates glycolysis by upregulating GLUT1 expression. A, heatmap shows expression proﬁles of the glycolysis-related genes in BT- 549 CD44v10 overexpression cells compared to BT-549 vector cells. Red colors indicate upregulation, and blue indicates downregulation. B, expression validation of candidate genes from heatmap by qPCR. The means ± SD of relative fold changes from triplicate experiments were plotted. β-actin was used as the control. The p values were calculated by paired Student’s t test. C, analysis of GLUT1 expression by Western blot in CD44v10 OE cells. D, knockdown efﬁciency of GLUT1 was evaluated by Western blotting in CD44v10 OE cells. E, the effects of GLUT1 on glucose consumption and lactate production in BT- 549 cells. F, the effects of GLUT1 on ROS levels in BT-549 cells. G, relative levels of phosphorylation of ERK, AKT, and GLUT1 proteins in BT-549 CD44v10 overexpression cells pretreated with ERK (U0126, 20 μM) or AKT inhibitor (LY294002, 20 μM). The ns indicates no signiﬁcance, **p <0.01, ***p <0.001. GLUT1, glucose transporter 1; ROS, reactive oxygen species.

Article Snippet: Then, a mouse anti-human CD44v10 (1:200, Bio-Rad, MCA1733) antibody was added, and the slides were incubated at 4 C overnight.

Techniques: Expressing, Over Expression, Plasmid Preparation, Biomarker Discovery, Control, Western Blot, Knockdown, Phospho-proteomics

Figure 5. CD44v10 promotes CSC properties of TNBC cells. A, the effects of CD44v10 overexpression or inhibition on sphere-forming ability were detected by sphere-forming assays. Scar bars, 400 μm for (4×) and 200 μm for (20×). B, the effects of CD44v10 overexpression or knockdown on protein expressions of a panel of stemness-related genes (Oct4, Klf4, c-Myc, ALDH1, and Nanog) were analyzed by Western blotting. C, ALDH-positive population was detected by ﬂow cytometry (left). The proportions were calculated from triplicate independent experiments (right). D and E, mRNA (D) and protein (E) levels of CD44v10 were tested in spheroid cells and adherent cells by qPCR (D) and Western blotting (E), respectively. F, the effects of GLUT1 on sphere- forming ability of BT-549 cells. Scar bars, 400 μm for (4×) and 200 μm for (20×). G, the effects of GLUT1 on the expressions of CSC-related genes in BT- 549 cells by Western blotting. *p <0.05, **p <0.01, ***p <0.001. ALDH, aldehyde dehydrogenase; CSC, cancer stem cell; GLUT1, glucose transporter 1; TNBC, triple-negative breast cancer.

Journal: The Journal of biological chemistry

Article Title: Cell adhesion molecule CD44v10 promotes stem-like properties in triple-negative breast cancer cells via glucose transporter GLUT1-mediated glycolysis.

doi: 10.1016/j.jbc.2022.102588

Figure Lengend Snippet: Figure 5. CD44v10 promotes CSC properties of TNBC cells. A, the effects of CD44v10 overexpression or inhibition on sphere-forming ability were detected by sphere-forming assays. Scar bars, 400 μm for (4×) and 200 μm for (20×). B, the effects of CD44v10 overexpression or knockdown on protein expressions of a panel of stemness-related genes (Oct4, Klf4, c-Myc, ALDH1, and Nanog) were analyzed by Western blotting. C, ALDH-positive population was detected by ﬂow cytometry (left). The proportions were calculated from triplicate independent experiments (right). D and E, mRNA (D) and protein (E) levels of CD44v10 were tested in spheroid cells and adherent cells by qPCR (D) and Western blotting (E), respectively. F, the effects of GLUT1 on sphere- forming ability of BT-549 cells. Scar bars, 400 μm for (4×) and 200 μm for (20×). G, the effects of GLUT1 on the expressions of CSC-related genes in BT- 549 cells by Western blotting. *p <0.05, **p <0.01, ***p <0.001. ALDH, aldehyde dehydrogenase; CSC, cancer stem cell; GLUT1, glucose transporter 1; TNBC, triple-negative breast cancer.

Article Snippet: Then, a mouse anti-human CD44v10 (1:200, Bio-Rad, MCA1733) antibody was added, and the slides were incubated at 4 C overnight.

Techniques: Over Expression, Inhibition, Knockdown, Western Blot, Cytometry

Figure 6. CD44v10 correlates with paclitaxel sensitivity of TNBC cells. A, the effects of paclitaxel (PTX) treatment on the protein expressions of CD44v10 and stemness-related genes in BT-549 and MDA-MB-231 cells by Western blotting. B, CCK-8 assay is used to evaluate the chemosensitivity of BT-549 and MDA-MB-231 cells to different concentrations of PTX after 48 h treatment. C, the effects of CD44v10 on the sensitivity of BT-549 and MDA-MB-231 cells to PTX by colony formation assay. D, the effects of GLUT1 on the sensitivity of BT-549 cells to PTX by CCK-8 assay. Statistical signiﬁcance was determined by one-way ANOVA. Data are shown as the mean ± SD. The ns indicates no signiﬁcance, *p <0.05, **p <0.01, ***p <0.001. GLUT1, glucose transporter 1; TNBC, triple-negative breast cancer.

Journal: The Journal of biological chemistry

Article Title: Cell adhesion molecule CD44v10 promotes stem-like properties in triple-negative breast cancer cells via glucose transporter GLUT1-mediated glycolysis.

doi: 10.1016/j.jbc.2022.102588

Figure Lengend Snippet: Figure 6. CD44v10 correlates with paclitaxel sensitivity of TNBC cells. A, the effects of paclitaxel (PTX) treatment on the protein expressions of CD44v10 and stemness-related genes in BT-549 and MDA-MB-231 cells by Western blotting. B, CCK-8 assay is used to evaluate the chemosensitivity of BT-549 and MDA-MB-231 cells to different concentrations of PTX after 48 h treatment. C, the effects of CD44v10 on the sensitivity of BT-549 and MDA-MB-231 cells to PTX by colony formation assay. D, the effects of GLUT1 on the sensitivity of BT-549 cells to PTX by CCK-8 assay. Statistical signiﬁcance was determined by one-way ANOVA. Data are shown as the mean ± SD. The ns indicates no signiﬁcance, *p <0.05, **p <0.01, ***p <0.001. GLUT1, glucose transporter 1; TNBC, triple-negative breast cancer.

Article Snippet: Then, a mouse anti-human CD44v10 (1:200, Bio-Rad, MCA1733) antibody was added, and the slides were incubated at 4 C overnight.

Techniques: Western Blot, CCK-8 Assay, Colony Assay

Journal: International Journal of Cell Biology

Article Title: Extracellular Vesicles from Caveolin-Enriched Microdomains Regulate Hyaluronan-Mediated Sustained Vascular Integrity

doi: 10.1155/2015/481493

Figure Lengend Snippet: Characterization of potential bioactive components in HA-induced EV. Panel (a): HPMVEC were grown to confluence and switched to serum-free media and either no HA (control), 100 nM HMW-HA, 100 nM LMW-HA, or 500 ng/mL LPS for 24 hours. EVs were then isolated, run on 4–20% TBE gels, and stained with Alcian blue. Purified enlargeosomes contained HA of ~1 million Da (see arrow) consistent with HMW-HA which we have previously demonstrated to be barrier enhancing [ , , , ]. In contrast, control, LMW-HA, or LPS-induced EV contained negligible HA. Human plasma was used as a control. Panel (b): HPMVEC were grown to confluence and switched to serum-free media and either no HA (control), 100 nM HMW-HA, or 100 nM LMW-HA for 24 hours. EVs were then isolated, run on SDS-PAGE, and immunoblotted with anti-CD44 (IM-7) (a), anti-CD44v10 (b), anti-HABP2 (c), anti-CD63 (d), or anti-AHNAK (e) antibodies. HMW-HA-induced enlargeosomes expressed the EC barrier enhancing CD44 isoform, CD44s (standard form) [ , ]. In contrast, LMW-HA-induced exosomes expressed the EC barrier disrupting HA binding proteins, CD44 isoform CD44v10, and the extracellular serine protease, HABP2 [ , ]. Basally secreted EV (control) had low expression of these molecules. Panel (c): isolated EVs as described in Panel (b) were subjected to RNA isolation and analysis (see ). Compared to control EV, LMW-HA-induced EV had less total RNA and microRNA while HMW-HA-induced enlargeosomes had ~2-fold higher levels of total RNA and microRNA.

Article Snippet: Antibodies utilized in this study include anti-CD9 (Santa Cruz Biotechnology Inc., Dallas, TX), anti-CD63 (Abcam, Cambridge, MA), anti-CD81 (GeneTex Inc., Irvine, CA), anti-AHNAK 1 (Thermo Scientific, Waltham, MA), anti-HABP2 (Abnova, Walnut, CA), anti-CD44 (IM7 clone) (BD Biosciences), anti-CD44v10 (Novus Biologicals), and anti-actin (Sigma).

Techniques: Isolation, Staining, Purification, SDS Page, Binding Assay, Expressing

Review

Structured Review

Images

1) Product Images from "Extracellular Vesicles from Caveolin-Enriched Microdomains Regulate Hyaluronan-Mediated Sustained Vascular Integrity"

Similar Products

Image Search Results